rabbit anti-mouse β2-ar antibody (Santa Cruz Biotechnology)
Structured Review

Rabbit Anti Mouse β2 Ar Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse β2-ar antibody/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Chronic psychosocial stress compromises the immune response and endochondral ossification during bone fracture healing via β-AR signaling"
Article Title: Chronic psychosocial stress compromises the immune response and endochondral ossification during bone fracture healing via β-AR signaling
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.1819218116
Figure Legend Snippet: Immune response in the fracture callus 3 d after femur osteotomy following 19 d of CSC housing. (A) Quantification of Ly6G+, (B) CD8+, and (C) F4/80+ cells in the fracture callus of SHC and CSC mice on d3 after fracture. (D) Representative image of fracture callus stained for Ly6G (scale bars: 50 µm), (E) CD8 (scale bars: 50 µm), and (F) F4/80 (scale bars: 50 µm) of SHC and CSC mice. (G) Relative gene expression of TH analyzed by qPCR from fracture hematoma homogenates. Values were normalized to B2M. (H) Representative image of fracture callus stained for TH in SHC and CSC mice on d3 after femur osteotomy. (Scale bars: 50 µm.) (I) Representative images of fracture calli from SHC (Left) and CSC (Right) mice on d3 after fracture, double-stained for TH and Ly6G: TH (Upper), Ly6G (Middle), merge (Lower). (Scale bars: 100 µm; inlay: 50 µm.) Data are presented as mean + SD. SHC, n = 4–7; CSC, n = 4–7. *0.05 > P > 0.01.
Techniques Used: Staining, Gene Expression
Figure Legend Snippet: Callus composition and vascularization on d10 after femur osteotomy following 19 d of CSC housing. (A) Bone area/total area (BA/TA) ratio, (B) cartilage area/total area (CA/TA) ratio, (C) fibrous tissue area/total area (FA/TA) ratio, and (D) whole callus area of SHC and CSC mice on d10 after fracture. (E) Representative staining of fracture calli stained with Safranin-O. (Scale bars: 500 µm.) (F) Relative gene expression of Runx2, VEGF, and Sox2 analyzed by qPCR following LCM of the cartilage-to-bone transition zone of the fracture callus. Values were normalized to B2M. (G) Representative image of fracture callus stained for Runx2 (scale bars: 50 µm), (H) VEGF (scale bars: 50 µm), and (I) Sox2 (scale bars: 100 µm) in SHC and CSC mice on d10 after fracture. (J) Relative vascular area of transition zone. (K) Representative image of Safranin-O staining of the transition zone in SHC and CSC mice on d10 after fracture. (Scale bars: 50 µm.) (L) Representative images of fracture calli from SHC and CSC mice on d10 after fracture, double stained for β2-AR and ENPP1: β2-AR (Left), ENPP1 (Middle), merge (Left). (Scale bars: 100 µm and inlay, 50 µm.) Data are presented as mean + SD. SHC, n = 5–8; CSC, n = 5–8. *0.05 > P > 0.01; **0.01 > P > 0.001.
Techniques Used: Staining, Gene Expression